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KMID : 1157520210170030117
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International Journal of Clinical Preventive Dentistry
2021 Volume.17 No. 3 p.117 ~ p.127
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A Study Comparing the Characteristics of Zinc Oxide Eugenol-Based and Mineral Trioxide Aggregate-Based Root Canal Sealers
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Lee Seok-Eun
Cho Ja-Won
Yoo Hyun-Jun
Lee Myung-Gu
Jeon Yeol-Mae
Kim Da-Hui
Park Hye-Won
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Abstract
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Objective: This study aimed to investigate the antimicrobial activity and cytotoxicity of zinc oxide eugenol (ZOE)-based and mineral trioxide aggregate (MTA)-based root canal sealers.
Methods: The root canal sealers used in this study were Tubli-Seal (a ZOE-based sealer) and ProRoot (an MTA-based sealer). The antimicrobial activity of ProRoot and Tubli-seal was assessed using a susceptibility assay using Enterococcus faecalis, Porphyromonas endodontalis, and Porphyromonas gingivalis. The cytotoxicity of the root canal sealers was investigated using MTT assay, real-time RT-PCR, and ELISA after the pulp cells were treated with sealers. Additionally, the components of the root canal sealers were analyzed using X-ray fluorescence.
Results: The MTA-based sealer showed stronger antimicrobial activity against E. faecalis than the ZOE-based sealer, and the antimicrobial activities of both sealers against P. endodontalis and P. gingivalis were not significantly different. The ZOE-based sealer showed strong cytotoxicity towards pulp cells and induced the expression and production of inflammatory cytokines. However, the MTA-based sealer did not affect the viability of pulp cells or significantly induce the expression of inflammatory cytokines such as TNF-α, IL-6, and IL-8. In the component analysis, the MTA-based sealer had more kinds of metal oxides than the ZOE-based sealer.
Conclusion: The MTA-based sealers had stronger antimicrobial activity against E. faecalis compared to the ZOE-based sealer, and did not show significant cytotoxicity towards pulp cells. Based on these results, MTA-based sealers may be a suitable material for root canal therapy.
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KEYWORD
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mineral trioxide, root canal, sealers, zinc oxide eugenol
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